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Surface Passivation Study This link lists the data taken for a study that investigates how the gliding motility assay is affected by changing the surface passivation with different types of bovine casein. All the data contained herein is CC0. Uncompressed movies can be found in the folders. False colored videos of the assay can be found below.

Open Data Videos


The below videos have had dead pixels removed and false colored using ImageJ's "Green Fire Blue" LUT. The videos are sped up to 30 frames/s from the original 5 frames/s. Each frame is exposed for 100 ms using a 100 W Hg lamp that is attached to the microscope. The Hg lamp power is reduced by 94% to prevent excessive heating and over illumination of the sample. The images are taken with a Luca S EMCCD camera that uses a gain of 150. The microtubules are tagged with rhodamine and are imaged by epifluorescence using a TRITC filter cube in an Olympus IX71 microscope. The objective is a 60x 1.42NA Plan Apo objective. The pixel size in the videos is 166.7 nm. The objective is also temperature stabilized and all data was taken with an observed temperature of the slide to be 33.1 +/- 0.1 degrees Celsius. To see a video of the assay, choose a playlist below and then choose a video from the video player. Surface passivation is done with 1 mg/mL casein.
Playlists Description
Alpha casein 01 Alpha casein 02
Alpha casein 03 Alpha casein 04 		These assays use 1 mg/mL alpha casein as the surface passivator.
Beta casein 01 Beta casein 02
Beta casein 02 		These assays use 1 mg/mL beta casein as the surface passivator.
Kappa casein 01 Kappa casein 02
Kappa casein 03 		These assays use 1 mg/mL kappa casein as the surface passivator.
Whole casein 01 Whole casein 02
Whole casein 03 Whole casein 04 		These assays use 1 mg/mL whole casein as the surface passivator.
Mixed casein 01 Mixed casein 02
Mixed casein 03 		These assays use 1 mg/mL of mixed casein as the surface passivator. The mixed casein is comprised of 49% alpha casein, 37% beta casein and, 14% kappa casein.